azino bis 3 ethylbenzthiazoline 6 sulfonic acid

Wang H., Zhao M., Yang B., Jiang Y., Rao G. Extraction, identification and characterization of the water-insoluble proteins from tobacco biomass. Relationship between boron and phenolic metabolism in tobacco leaves. Ruiz J.M., Bretones G., Baghour M., Ragala L., Belakbir A., Romero L. Extraction of essential oil from discarded tobacco leaves by solvent extraction and steam distillation, and identification of its chemical composition. Zhang X., Gao H., Zhang L., Liu D., Ye X. Thus, tobacco leaves could be considered as a potential source of natural antioxidants for food, pharmaceutical, cosmetics or nutraceutical industries. These results clearly indicate that flavonoids are effective in scavenging free radicals and have the potential to be powerful antioxidants. Meanwhile, it was found that flavonoids had prominent effects on the reducing power, which was equivalent to ascorbic acid, and was significantly higher than polysaccharides. However, flavonoids possessed comparable superoxide anion, DPPH and ABTS radical scavenging abilities to ascorbic acid at high concentration (600 μg/mL). When compared to the positive control, ascorbic acid, both showed weaker antioxidant potential.

Flavonoids showed much better activity than polysaccharides in scavenging activities on free radicals. In the present study, antioxidant properties of flavonoids and polysaccharides from tobacco (Nicotiana tabacum L.) leaves were evaluated in several in vitro systems, e.g., scavenging activities on hydroxyl, superoxide anion, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radicals, and reducing power.

Azino bis 3 ethylbenzthiazoline 6 sulfonic acid